Isolation and Purification of Proteins
by Hatti-Kaul, Rajni; Mattiasson, BoPub. Date: 2003-02-05
Publisher(s): Taylor & Francis
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Summary
This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Table of Contents
| Resolution technique for protein separation | |
| Selectivity in affinity chromatography | |
| Genetic approaches to facilitate purification | |
| solid-liquid separation | |
| Membranes for protein isolation and purification | |
| Precipitation of proteins - non-specific and specific | |
| Protein crystallization for large-scale bioseparation | |
| Extraction for rapid protein isolation | |
| Adsorption as an initial step for the capture of proteins | |
| Fast chromatography of proteins | |
| Novel approaches to the chromatography of proteins | |
| Scale up and commercial production of recombinant proteins | |
| Process monitoring in downstream processing | |
| Protein formulation | |
| Regulatory requirements | |
| Cleaning and sanitization issues in protein purification | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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