Fluorescence Probes in Oncology

by Kohen, Elli; Hirschberg, Joseph G.; Santus, Rene

ISBN13: 9781860941504

ISBN10: 1860941508

Format: Hardcover

Pub. Date: 2002-07-01

Publisher(s): World Scientific Pub Co Inc

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Summary

This book is unique in the sense that it will provide a comprehensive description of fluorescence probes and the methodologies applicable to the study and diagnostics in oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology and provides a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad portfolio of spectral and fluorescence imaging which most appropriately supplement the information derived from Virchow style microscope slides (they still remain valid after more than 150 years, but a considerable body of knowledge and interpretation can be built around them). Following an introductory section are sections on Fundamentals, Methods and Instrumentation, Fluorescent Probes, and New Method Applications. The final section is dedicated to Applications in Cellular Physiopathology.

Preface

vii

Introduction

Acknowledgments

The Nature of Light

(20)

Origins

(1)

Refraction

(2)

Fermat's Principle

(1)

The Speed of Light

(1)

The Wave Nature of Light

(5)

What Are Waves?

(1)

Wave Characteristics

(4)

Diffraction and Resolution Limits

(3)

The Quantum Theory and Photons

(3)

The Production of Light

(4)

The Energy of Bound Electrons

(4)

Electron Spin

(1)

The Planck Equation and Photochemical Reactions

(2)

References

(1)

Introduction to Photophysics

(24)

From Atoms to Molecules: The Formation of Chemical Bonds

(2)

Electronic Transitions

(2)

Excitation and Deactivation of Molecules

(12)

The Jablonski Diagram

(1)

The Excitation Coefficient: The Beer-Lambert Law

(2)

The Franck-Condon Principle

(1)

Deactivation from Singlet Excited States

(1)

Fluorescence Excitation Spectrum

(1)

Excitation-Emission Matrices

(3)

Deconvolution of Spectra

(2)

Deactivation of the First Singlet Excited State by Intersystem Crossing to the Triplet State

(2)

Factors Influencing the Fluorescence Quantum Yield

(3)

Definition of the Fluorescence Quantum Yield

(1)

Sensitivity of Fluorescence to Environment

(1)

Impurity Quenching of Fluorescence: The Stern-Volmer Equation

(1)

Quenching of the First Excited Singlet State By Energy Transfer

(5)

Energy Transfer Between Singlet States

(3)

References

(2)

Basic Approaches to the Experimental Study of Chemical and Biological Luminescence

(24)

Long-Lifetime Probes: Metal-ligand Complexes as a New Class of Long-Lived Fluorophores for Protein Hydrodynamics

(12)

References

(1)

Chemiluminescence

(1)

Reference

(1)

Bioluminescence

(3)

References

(1)

Green Fluorescent Protein (GFP)

(8)

References

(1)

Methods and Instrumentation

(68)

Spectrofluorometers and Cell Sorters

(2)

Reference

(1)

Microspectrofluorometers

(3)

References

(1)

Detectors Used in Fluorescence Imaging

(3)

Charge-Coupled Devices: CCDs

(1)

Reference

(1)

Avalanche Diodes

(2)

Reference

(1)

Fluorescence Imaging with CCD Cameras

(1)

Digital Imaging

(2)

Reference

(1)

Ratio Fluorescence Microscopy

(12)

References

(1)

Multiparameter Imaging

(3)

References

(1)

Multiprobe Approach to the Study of Intercellular Communication

(7)

References

(7)

Photodiode Array Fluorescence Spectroscopy

101

(1)

Confocal Fluorescence Microscopy

102

(7)

References

103

(1)

Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens

103

(2)

References

105

(1)

Confocal 3-D Volume Investigation

105

(2)

Reference

107

(1)

Fluorophores for Confocal Microscopy

107

(1)

Reference

108

(1)

Biological Applications of Confocal Microscopy

108

(1)

Fluorescence Lifetime Imaging (FLIM)

109

(8)

References

116

(1)

Frequency-Domain Spectroscopy and Tomography of Tissues

117

(2)

Fluorescence Lifetime Spectroscopy and Imaging in Scattering Media with Frequency-Domain Techniques

117

(2)

References

119

(1)

Fluorescence Photobleaching Recovery (FPR, Also FRAP) and Green Fluorescent Protein (GFP) Chimeras

119

(6)

Reference

125

(1)

Time-Resolved Fluorescence Resonance Energy Transfer (FRET)

125

(1)

Reference

126

(1)

Fluorescence Polarization in the Microscope

126

(2)

Reference

128

(1)

Polarization Fluorescence Photobleaching Recovery (PFPR)

128

(1)

Picosecond Fluorescence Kinetics

129

(1)

Reference

130

(1)

Raman Fluorescence Spectroscopy in Single Living Cells

130

(3)

References

133

(1)

Fluorescence Probes in Oncology

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