Antigen Retrieval Immunohistochemistry Based Research and Diagnostics
by Shi, Shan-Rong; Taylor, Clive R.Edition: 1st
Format: Hardcover
Pub. Date: 2010-09-28
Publisher(s): Wiley
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Summary
This book is based on the development and application of AR by the editors, one of whom is the inventor of AR, together with members of a world-leading research center of AR. Contributions are made by a worldwide group of leading research scientists to summarize recent achievements in AR-IHC including numerous cutting edge issues for future research projects. Thus, this book is aimed not only at all practitioners in morphology, but also research scrientists who represent a wide spectrum of interest.
Author Biography
Shan-Rong Shi, MD, is Professor of Clinical Pathology at the Keck School of Medicine of the University of Southern California. He is the Associate Editor of the Journal of Histochemistry and Cytochemistry, serves on the Editoral Board of Applied Immunohistochemisty and Molecular Morphology, and is also on the Overseas Editorial Board of the Chinese Journal of Pathology. Dr. Shi is the inventor of the antigen retrieval technique. Clive R. Taylor, MD, PhD, is Professor and former chairman of pathology, and Senior Associate Dean at the Keck School of Medicine. He is also Editor in Chief of Applied Immunohistochemistry and Molecular Morphology. Dr. Taylor is a world-renowned expert in immunohistochemistry and clinical pathology.
Table of Contents
| Preface | p. ix |
| Contributors | p. xv |
| Recent Advances in Antigen Retrieval Techniques and Its Application | p. 1 |
| Standardization of Antigen Retrieval Techniques Based on the Test Battery Approach | p. 3 |
| Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in Situ Hybridization | p. 25 |
| Extraction of DNA/RNA from Formalin-Fixed, Paraffin-Embedded Tissue Based on The Antigen Retrieval Principle | p. 47 |
| Standardization of Immunohistochemistry | p. 73 |
| Key Issues and Strategies of Standardization for Quantifiable Immunohistochemistry | p. 75 |
| Standardization of Immunohistochemistry Based on Antigen Retrieval Technique | p. 87 |
| Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of HER2 IHC and ISH Testing | p. 101 |
| Peptides as Immunohistochemistry Controls | p. 123 |
| Standard Reference Material: Protein-Embedding Technique and Design of Bar Code | p. 141 |
| The Pros and Cons of Automation for Immunohistochemistry from the Prospective of the Pathology Laboratory | p. 151 |
| Image Analysis in Immunohistochemistry | p. 165 |
| Tissue/Cell Sample Preparation | p. 187 |
| Tissue Cell Sample Preparation: Lessons from the Antigen Retrieval Technique | p. 189 |
| Mechanisms of Action and Proper Use of Common Fixatives | p. 195 |
| Cell Sample Preparation for Clinical Cytopathology: Current Status and Future Development | p. 219 |
| Design of a Tissue Surrogate to Examine Accuracy of Proteomic Analysis | p. 235 |
| Molecular Mechanism of Antigen Retrieval Technique | p. 251 |
| Study of Formalin Fixation and Heat-Induced Antigen Retrieval | p. 253 |
| A Linear Epitopes Model of Antigen Retrieval | p. 287 |
| pH or Ionic Strength of Antigen Retrieval Solution: A Potential Role for Refolding during Heat Treatment | p. 303 |
| Commentary: Future Directions | p. 323 |
| Proteomic Analysis of Protein Extracted from Tissue/Cells | p. 333 |
| Techniques of Protein Extraction from FFPE Tissue/Cells for Mass Spectrometry | p. 335 |
| Application of Shotgun Proteomics To Formalin-Fixed and Paraffin-Embedded Tissues | p. 347 |
| Visualizing Protein Maps in Tissue | p. 369 |
| Symbiosis of Immunohistochemistry and Proteomics: Marching to a New ERA | p. 391 |
| Appendix: Related Laboratory Protocols | p. 399 |
| Index | p. 413 |
| Table of Contents provided by Ingram. All Rights Reserved. |
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